#Flowjo facs for mac#

In case of sarcoma 45, intraperitoneal injection of copper nanoparticles was more effective than intratumoral one, while in rats with Pliss' lymphosarcoma intratumoral injection of the nanoparticles had some advantages. The ICF provides access to this software: FlowJo for MAC and PC FlowJo dongle for short-term loan (book online) BD Diva Software Flowing Software Two. The efficiency depended on the approach of nanoparticles injection.Ĭonclusion. In the case of a rapidly growing tumor model (Pliss' lymphosarcoma), a decrease in tumor growth rates caused by copper nanoparticles occurred at later stages of exposure, after the animals received nanoparticles at a total dose of 5–10 mg/kg. Experiments on a transplantable, slowly growing sarcoma 45 showed a decrease in the specific tumor growth rate after a single injection of nanoparticles (1.25 mg/kg), a decrease in tumor size could be observed after 4 injections (a total dose of 5 mg/kg). The effects of nanodispersed copper differed in tumors with different histological structures and growth patterns. In most animals (up to 89 %), significant antitumor effects of copper nanoparticles were obtained up to complete regression of tumors with large volumes. Introduction Traditional Compensation in FlowJo FlowJo Media 4.89K subscribers Subscribe 28K views 2 years ago Find more FlowJo tutorials here. Contrary to other researches, we started the exposure to metal nanoparticles only when the tumors had become visible and had grown up to the sizes at which their spontaneous regression was unlikely. Nanoparticles had a 'core-shell' structure with variable diameter in the range of 30–75 nm. In experiments on 163 white outbred male rats, 160–200 g, the size and growth rate of transplanted sarcoma 45 and Pliss' lymphosarcoma were determined in dynamics when a suspension of copper nanoparticles in physiological saline was injected intratumorally or intraperitoneally in a total dose of 10 mg/kg. To study the effect of copper nanoparticles on the characteristics of the transplanted tumors of rats, as well as to assess the dependence of the effect on the method of administration of nanoparticles.

#Flowjo facs free#

This service is currently free of charge, too.Purpose of the study. If you are sorting in the CFFC you will receive step-by-step tuition and guidance leading you to being able to work with the FACSAria sorter independently and operator-free. General Good Practices for Flow Cytometry and Cell Sorting Titrate your antibodies (and any other reagents) to maximize the resolution of your staining Verify. Recent advances in fluorescence-activated cell sorting (FACS) technology offer new and exciting approaches for understanding, monitoring and combating immune-related diseases. of single-cell research projects that utilize flow cytometry. This workshop is co-organized by TransMed and the Core Facility Flow Cytometry (CFFC)Ībout the Core Facility Flow Cytometry (CFFC)Įveryone is welcome to use FlowJo TM on the CFFC workstations, currently free of charge. Sign up for our free webinar, Intro to FlowJo v10 Thursday, February 2nd at 10:00 a.m. Please bring your own data with you in order to analyze them.įor the workshop 1 CP for scientific skills training can be credited (0.5 CP for participating only in the theoretical part). Please read the FlowJo TM description, which will be sent to you after your registration. Theoretical and practical input by the trainer, showing examples, analyzing own data using FlowJo TM with the help of the trainer, discussing own issues, feedback from the trainer.ĭoctoral students, postdocs and clinician scientists who already know the basics of FlowJo TM. Using R-independent plugins with FlowJo TM.Using R-dependent plugins with FlowJo TM.Editing an existing compensation matrix.Creating a compensation matrix using the Wizard.Copies may be purchased for laboratories internal to the. Heat mapping statistics in the Table and Layout Editors Cell sorting on our FACS Aria IIIu or Influx requires the skill of a trained operator.Overlays - grouping by panel or keyword.General overview of FlowJo TM including the following:.There is enough time to discuss specific issues. In the afternoon, participants practice FlowJo in pairs using own data. In the morning, the software is explained theoretically using examples.

#Flowjo facs how to#

This beyond the basic workshop shows participants how to analyze flow cytometry data using FlowJo TM.